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Bioreactors for Stem Cell Expansion and Differentiation .

Bioreactors for Stem Cell Expansion and Differentiation .

An international team of investigators presents thought-provoking reviews of bioreactors for stem cell expansion and differentiation and provides cutting-edge information on different bioreactor systems. The authors offer novel insights into bioreactor-based culture systems specific for . pluripotent stem cells 149. vivo 148. vitro 148. shear .

Current understanding and challenges in bioprocessing of .

Current understanding and challenges in bioprocessing of .

However, the system was unsuitable for cell-based therapy bioprocessing due to the considerable risk for well-to-well cross-contamination, and being unlikely to achieve required therapeutic scale. 6, 7 Last year, several reports demonstrated that hESCs can be cultured in suspension without microcarrier attachment, 9 thereby possibly suggesting .

Advances in cell culture: anchorage dependence .

Advances in cell culture: anchorage dependence .

Where stem cells are grown as aggregates, attached to microcarriers or immobilized in scaffolds, suspension culture systems are employed. A reactor system (stirred-tank reactor) is the culture system of choice when an unlimited scale-up under controlled conditions is required.

Hollow Fiber Bioreactors for In Vivo-like Mammalian Tissue .

Hollow Fiber Bioreactors for In Vivo-like Mammalian Tissue .

Summary. The functional behavior of cells in culture can be improved by culturing in more in vivo-like 3-dimensional culture environments 16-21.This manuscript describes the set-up and operation of a hollow fiber bioreactor system for in vivo-like mammalian tissue culture.

Mesenchymal stromal cell therapy: progress in .

Mesenchymal stromal cell therapy: progress in .

Mesenchymal stromal cell (MSC) therapies have been pursued for a broad spectrum of indications but mixed reports on clinical efficacy have given rise to some degree of skepticism regarding the effectiveness of this approach. However, recent reports of successful clinical outcomes and regulatory approvals for graft-versus-host disease, Crohn's disease and critical limb ischemia have prompted a .

Hollow Fiber Bioreactors for In Vivo-like Mammalian Tissue .

Hollow Fiber Bioreactors for In Vivo-like Mammalian Tissue .

Tissue culture is an established technique for the growth and/or maintenance of cells that has been utilized for over 100 years 1,2.The convenience of studying cells and responses ex vivo has far reaching advantages allowing experiments that would otherwise be extremely challenging if not impossible, for example the generation of genetically engineered cell lines and the use of reporter cells .

Optimizing the production of suspension cells using the G .

Optimizing the production of suspension cells using the G .

Broader implementation of cell-based therapies has been hindered by the logistics associated with the expansion of clinically relevant cell numbers ex vivo. To overcome this limitation, Wilson Wolf Manufacturing developed the G-Rex, a cell culture flask with a gas-permeable membrane at the base that supports large media volumes without compromising gas exchange.

Human-Induced Pluripotent Stem Cell Technology and .

Human-Induced Pluripotent Stem Cell Technology and .

Human-induced pluripotent stem cells (hiPSCs) are reprogrammed cells that have hallmarks similar to embryonic stem cells including the capacity of self-renewal and differentiation into cardiac myocytes. The improvements in reprogramming and differentiating methods achieved in the past 10 years widened the use of hiPSCs, especially in cardiac research. hiPSC-derived cardiac myocytes (CMs .

Bioprocess development for the cell-based treatment of .

Bioprocess development for the cell-based treatment of .

The widespread cellular treatment of type 1 diabetes by islet transplantation is limited by tissue shortage and graft rejection. This work describes two novel bioprocesses to immobilize pancreatic cells in alginate using: (1) hollow fiber bioreactors or (2) alginate bead generation by an adapted emulsion and internal gelation process. After optimization, live cell recovery rates and growth .

Bioreactors for Stem Cell Expansion and Differentiation .

Bioreactors for Stem Cell Expansion and Differentiation .

An international team of investigators presents thought-provoking reviews of bioreactors for stem cell expansion and differentiation and provides cutting-edge information on different bioreactor systems. The authors offer novel insights into bioreactor-based culture systems specific for . pluripotent stem cells 149. vivo 148. vitro 148. shear .

Defining Optimum Conditions for the . - Stem Cells Journals

Defining Optimum Conditions for the . - Stem Cells Journals

Nevertheless, contact might be necessary to preserve in culture the pluripotent, perhaps even CD34 − stem cell. This pre‐stem cell might need intermittent cell‐cycle arrest in a niche provided by stroma cells to perform asymmetric division and thereby enabling the hematopoiesis to be regenerated for an individual life span.

10.1016/j.bej.2013.11.014 | 10.1016/j.bej.2013 . - DeepDyve

10.1016/j.bej.2013.11.014 | 10.1016/j.bej.2013 . - DeepDyve

In fact, aeration is also a very important parameter since it provides oxygen and CO 2 (for pH control) to the culture system. In particular for stem cell cultivation, oxygen is known to have an important impact not only on cell growth and metabolism, but also on cell phenotype. . Stem Cells (mESC) production, as a model of pluripotent stem .

Bioreactor Engineering Fundamentals for Stem Cell .

Bioreactor Engineering Fundamentals for Stem Cell .

2016-1-1 · Table 3.1 lists the many different physical aspects of stirred bioreactors that need to be understood and considered for bioreactors containing low, constant viscosity, μ (Newtonian), media in which cells are grown in free suspension regardless of the cell type or scale ; the special additional features associated with cultivation on microcarriers are highlighted in bold.

Bioprocess for producing uniform embryoid bodies from .

Bioprocess for producing uniform embryoid bodies from .

2009-6-16 · FIG. 9 is a series of flow cytometry plots showing phenotypic detection of endothelial cells generated from encapsulated ES cells grown in stirred suspension bioreactors using flow cytometric based cell surface expression of various endothelial cell markers. FIG. 9 panel A shows cells expressing Flk-1 (y-axis) and CD34 (x-axis).

The Use of Multidimensional Image-Based Analysis to .

The Use of Multidimensional Image-Based Analysis to .

2011-10-20 · The transition from traditional culture methods towards bioreactor based bioprocessing to produce cells in commercially viable quantities for cell therapy applications requires the development of robust methods to ensure the quality of the cells produced. Standard methods for measuring cell quality parameters such as viability provide only limited information making process monitoring and .

Stem cell bioprocessing for regenerative medicine, Journal .

Stem cell bioprocessing for regenerative medicine, Journal .

Stem cell‐derived products have the potential to represent promising therapeutic approaches for the treatment of a wide range of conditions. Neurodegenerative diseases, like Parkinson's disease or Huntington's disease, neurological disorders, cardiac failure, and blood disorders, among others, may one day be treated using cellular therapies and regenerative medicine approaches based on stem .

Large-scale production of megakaryocytes in microcarrier .

Large-scale production of megakaryocytes in microcarrier .

2018-7-5 · Megakaryocytes (MKs) are the precursors of platelets (PLTs) and may be used for PLT production in vivo or in vitro, as well as a source for PLT-derived growth factors. Induced pluripotent stem .

Bioprocessing Strategies for Pluripotent Stem Cells Based .

Bioprocessing Strategies for Pluripotent Stem Cells Based .

As increasing numbers of cell-based therapies advance through clinical trials towards approval for use in humans, the need for robust and well-characterized cell culturing strategies is becoming increasingly apparent. We discuss here novel stem cell bioprocessing strategies based on the concept of using Waddington's epigenetic landscape to represent the process of cellular decision-making .

Single Use Bioreactor - Lonza Ltd

Single Use Bioreactor - Lonza Ltd

2017-12-7 · 1. A bioreactor comprising: a bioprocess container made from a liquid impermeable and flexible shape-conforming material, the bioprocess container having a top, a bottom, and at least one side wall therebetween, the bioprocess chamber defining a hollow enclosure for receiving a culture media; and a mixing device comprising a rotatable shaft coupled to at least one top impeller and at least one .

Microparticles for Suspension Culture of Mammalian Cells .

Microparticles for Suspension Culture of Mammalian Cells .

2019-7-15 · The focus of this work is to develop a technology for the synthesis of polymer microcarriers that demonstrate mammalian cell culture adhesion on the surface of the microcarriers. Most mammalian cells are adherent in nature that requires multilayer vessels, large volume, expensive cell culture media, high manufacturing time, and high costs of cell culture supplies for the commercial-scale .

Microparticles for Suspension Culture of Mammalian Cells .

Microparticles for Suspension Culture of Mammalian Cells .

2019-7-15 · The focus of this work is to develop a technology for the synthesis of polymer microcarriers that demonstrate mammalian cell culture adhesion on the surface of the microcarriers. Most mammalian cells are adherent in nature that requires multilayer vessels, large volume, expensive cell culture media, high manufacturing time, and high costs of cell culture supplies for the commercial-scale .

Optimizing the production of suspension cells using the G .

Optimizing the production of suspension cells using the G .

Identifying the maximum cell numbers per surface area in the G-Rex. We have previously demonstrated that the expansion of antigen-specific T cells, natural killer cells, regulatory T cells, and cell lines in G-Rex devices is superior to that achieved in conventional tissue culture–treated plates or gas-permeable bags. 12,13,20–24 This is due to the increased cell viability and improved .

Inertial-Based Filtration Method for Removal of .

Inertial-Based Filtration Method for Removal of .

2018-8-20 · Among different adherent cell bioreactors, employing suspension scaffolds so-called microcarriers (MCs), ~100–300 µm in diameter, within a stirred .

Stirred suspension bioreactors as a novel method to enrich .

Stirred suspension bioreactors as a novel method to enrich .

Cell suspensions were placed in stirred suspension culture, after 18, 44, and 66 h cell suspensions were filtered through a 40 μ m strainer to remove larger cell aggregates and poured back into culture until the next time point. 2B. Enrichment for germ cells by differential plating. 2C.

Impact of stirred suspension bioreactor culture on the .

Impact of stirred suspension bioreactor culture on the .

Tumorigenicity of bioreactor-derived cardiomyocytes. The elegant study by Zandstra et al., demonstrated that ESC-derived cardiomyocytes could indeed be generated in a scalable stirred suspension bioreactor provided cells were lineage selected [].However, based on our previous observation demonstrating increased tumorigenesis of ESC-derived chondrocytes and osteoblasts in stirred suspension .

Current understanding and challenges in bioprocessing of .

Current understanding and challenges in bioprocessing of .

However, the system was unsuitable for cell-based therapy bioprocessing due to the considerable risk for well-to-well cross-contamination, and being unlikely to achieve required therapeutic scale. 6, 7 Last year, several reports demonstrated that hESCs can be cultured in suspension without microcarrier attachment, 9 thereby possibly suggesting .

Optimization of agitation speed in spinner flask for .

Optimization of agitation speed in spinner flask for .

Scalable stirred-suspension bioreactor culture of human pluripotent stem cells. Tissue Eng Part A. 2010; 16:405–421. doi: 10.1089/ten.tea.2009.0454. [PMC free article] Krawetz R, Taiani JT, Liu S, Meng G, Li X, Kallos MS, Rancourt DE. Large-scale expansion of pluripotent human embryonic stem cells in stirred-suspension bioreactors.

Effect of microgravity on proliferation and .

Effect of microgravity on proliferation and .

Establishment of an automated culture system to culture mouse embryonic stem cells (mESCs) and embryoid bodies (EBs). A, In our cell culture chamber, real‐time growth of cells can be monitored by microscopy through the window (white arrow). B, Pictures of the medium storage bags (red dotted line) and waste bags (white dotted line).

A fully defined static suspension culture system for large .

A fully defined static suspension culture system for large .

2018-8-30 · Here, we report on an efficient large-scale culture system, which enables long-term, GMP grade, single-cell inoculation, and serial expansion of hESCs with a yield of about 1.5 × 10 9 cells per 1 .

Optimizing the production of suspension cells using the G .

Optimizing the production of suspension cells using the G .

Broader implementation of cell-based therapies has been hindered by the logistics associated with the expansion of clinically relevant cell numbers ex vivo. To overcome this limitation, Wilson Wolf Manufacturing developed the G-Rex, a cell culture flask with a gas-permeable membrane at the base that supports large media volumes without compromising gas exchange.